*At the prompt, type “rga” and wait
for the message "rga finished"
before proceeding to the next command.
*At the prompt type “ns
#”, where
# is the number of scans. (To see other acquisition
parameters it is helpful to type “eda” and parameters
will
be displayed)
*At the prompt, type “zg”, after spectrum is acquired type
“bc”
(baseline correction),
“em (exponential multiplication), “ft” (Fourier
transformation)and
“apk” (automatic
phasing procedure). For a better result you might
like
to perform a manual phase
correction and use additional baseline correction
subroutine
“abs” or other (Bruker
offers variety of such subroutines).
* Click on “Calibrate”, select
solvent
peak or TMS with the middle mouse button,
and type in the solvent chemical shift (reference
table
is on the bulletin board)
* If you need to perform integration, make sure that
baseline
is flat and click
“Integrate” button.
* Use the left mouse button to get the cursor, and the
middle
to define the integrating
region. Adjust “slope
” and “bias” if needed. Click “calibrate” to set up the integral
reference.
* Click “Return”, and then “
save integral and return”
* Type “edg”, and edit the
various
display and plotting parameters, if needed
* Type "setmi" and
adjust the treshold above noise.
* Type “view’, and
make sure the
plot looks right (adjust peak height with “CY
”
command.
* Type “plot”
* After experiment is completed: Press “
lock ON/OFF” and “spin ON/OFF”
* In the lock window, click “quit
”
* To retrieve the sample, press “lift ON/OFF
” again,
* At the xwinnmr prompt type exit
* From the UNIX window click log out of the Sgi workstation
* Make sure the none of light “lock
”,”spin” or “lift
” lit when you leave.
* Insert the plastic cap on top of the sample insertion port.
* Record your time in the logbook
There is a more extensive version of this manual. Contact
lab
manager if you are interested.